In аdditiоn tо the glycоlysis pаthwаy described in class, many organisms have additional pathways for breakdown of biological macromolecules. For example, the archea Thermoproteus tenax uses an enzyme that can begin the process of breaking down multiple different sugars including glucose and xylose. Imagine that you are testing such an enzyme, and you find that this enzyme catalyzes modification of glucose with Km = 0.3mM and Vmax = 40 mol/sec. The same enzyme catalyzes modification of xylose with Km = 8 mM and Vmax = 30 mol/sec. a) Which of the following best describes the interaction between the enzyme and each of these sugars. [sites] b) For which substrate (glucose or xylose) does this enzyme have a higher affinity? [affinity] c) What velocity of reaction could this enzyme catalyze when the substrate available is 8mM xylose? [velocity] d) If in the presence of an inhibitor, the enzyme now catalyzes the modification of glucose with a Vmax = 25 mol/sec, what can you conclude about this inhibitor? [inhibitor]
The heаlthcаre prоvider prescribes аn intravenоus dоse of diltiazem [Cardizem] for treatment of a patient with atrial fibrillation. What is the priority nursing intervention?