A certаin X-linked dоminаnt diseаse in humans оccurs when there are mоre than 40 repeats of a CGG trinucleotide in a particular gene. 5' CAGTATGCA------(CGG)n-------ATGCGTAAT 3' 3' GTCATACGT------(GCC)n-------TACGCATTA 5' Where n refers to the number of CGG repeats. In the lab freezer, you find PCR primers that are complementary to the sequence on either side of the repeats, which will allow you to amplify DNA fragments containing the repeat. Shown below are the outline of a pedigree for the disease described above, and a representation of a gel showing PCR-amplified fragments detecting the number of CGG repeats in 8 siblings. The DNA corresponding to each individual is directly below his or her place in the pedigree. Which individuals are male? Which individuals are female? Which individuals are affected?
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