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In the liver, the first step in glycolysis is not catalyzed…
In the liver, the first step in glycоlysis is nоt cаtаlyzed by hexоkinаse, but by glucokinase. These enzymes catalyze the same reaction, but the Km of hexokinase is 0.1 mM while the Km for glucokinase is 5 mM. Upload an image of your answers to this question. Knowing this, under identical conditions and with the same concentration of the glucose substrate, which enzyme, hexokinase or glucokinase, produces product at the highest reaction rate? Briefly justify your answer. Assume that both enzymes have similar Vmax values. (2 pts.) Michaelis-Menton kinetics assumes a two-step reaction process. Given the data above, which of the two steps in the process most likely differs for the two enzymes. Briefly justify your answer. (2 pts.) The value of kcat for hexokinase is reported to be 28/sec. In the presence of 0.02 mM of the compound known as LY-2121260 it is reported to be 75/sec. Describe the effect LY-2121260 has on the overall enzymatic activity of hexokinase and state whether the Km or the Vmax of hexokinase is most affected by this compound. (2 pts.) Sketch and label a Lineweaver-Burke plot of initial velocity, Vo, vs. [S] for the enzyme glucokinase relative to the enzyme hexokinase. Note: This is a relative plot (not exact) to show the difference in activity of the two enzymes compared to each other. Assume that both enzymes have similar Vmax values. (3 pts.) Sketch and label a second Lineweaver-Burke plot of initial velocity, Vo, vs. [S] that compares hexokinase with and without a hypothetical uncompetitive inhibitor. Note: This is a relative plot (not exact) to show the difference in activity of the enzyme with and without the inhibitor. (3 pts.)
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