Whаt is the cоnfirmаtоry methоd for meаsuring drugs of abuse?
Sоlve this prоblem оn pаper, document your аpproаch and provide intermediate results. Report the final answers with 3 significant figures. Scan the pages(s) and upload them for grading. In a hypothetical scenario, artificial intelligence generative modeling tools have designed a new cyclic peptide P that inhibits an enzyme E that is involved in a serious disease. In cells, the total concentration of enzyme E is 0.15 nM (nM = nanomoles per liter). The cyclic peptide concentration that gets delivered into the cell is 1 nM. Experiments have shown 95% of all of the enzyme is inhibited by the peptide. Using this information, calculate: A) The binding constant KB of the peptide P binding to enzyme E. B) The binding free energy ΔG (assuming the temperature in cell is 37 Celsius degrees). C) Now let's assume that we would like to design a new peptide P*, such that when delivered at the same concentration as the peptide P, it would successfully inhibit 99.9% of the enzyme E. What would the binding constant of P* binding to E need to be? And what would the binding free energy need to be? D) Let's assume that in patients with a specific mutation, there is a different variant of the enzyme that we call "M" in their cells. It turns out that the peptide P is weaker at binding to M (which is present at concentration 0.15 nM) . The binding constant of P to M is measured to be Kb = 107. Calculate the concentration of peptide P that would be needed to inhibit 90% of enzyme M.
У меня болит голова и температура. Мне не надо (работать-поработать).